Bioprospecting bacterial strains for biological control of white mold on soybean

H. F. Shiomi, M. V. R. Ferreira, I. S. Melo

Resumo


 The search for efficient biocontrol agents in agriculture has been achieved in several pathosystems. Thus, we carried out experiments under controlled conditions in greenhouse and laboratory, involving the use of bacteria Bacillus alcalophilus, Bacillus cereus GC subgrupo B, Stenotrophomonas maltophilia, Yersinia bercovieri and Photorhabdus luminescens-luminescens obtained from biofertilizers and bovine and suine manure. The bacterial strains were multiplied in nutrient-agar medium (NA) for 48 hours to evaluate the efficiency in inhibiting the mycelial growth and the development of Sclerotinia sclerotiorum in antagonism assays in Petri dishes containing BDA medium; or in aqueous suspension adjusted in 109 ufc.mL-1 by Mac Farland scale, to seeds microbiolization and spraying the aerial parts of soybean plants, totalizing three assays. The bacterial strains BB-4 (Bacillus cereus GC subgrupo B), BS-3 (Photorhabdus luminescens-luminescens), BB-1 (Bacillus alcalophilus) and BB-6 (Yersinia bercovieri) tested were shown to be effective for inhibition of mycelial growth of the pathogen, with values between 31% and 46%, and disease development in soybean seeds and plants, with values above 50% and the same levels of control treatments with thiophanate methyl + fluazinan and thiophanate methyl. These results demonstrate the presence of promising strains in control of white mold alternatively the use of chemical fungicides


Palavras-chave


controle alternativo, Sclerotinia sclerotiorum, biocontrole

Texto completo:

PDF

Referências


ALMEIDA, A.M.R.; FERREIRA, L.P.; YORINORI, J.E.V.; SILVA, J.F.V.; HENNING, A.A. Doenças da soja (Glycine Max). In: KIMATI, H.; AMORIM, L.; REZENDE, J.A.M.; BERGAMIN FILHO, A.; CAMARGO, L.E.A. (Eds.) Manual de fitopatologia. Vol. 2. Doenças das Plantas Cultivadas. 4ª Ed. São Paulo SP, Ceres. p.565-568, 2005.

BAL, S.; MISHRA, R.R.; RATH, B.; SAHU, H.K.; TRATOI, H.N. Characterization and extracellular enzyme activity of predominant marine Bacillus spp. isolated from sea water of Orissa Coast, India. Malaysian Journal of Microbiology, Penang, 5 (2):87-93, 2009.

BETTIOL, W. Controle de doenças de plantas com agentes de controle biológico e outras tecnologias. In: CAMPANHOLA, C.; BETTIOL, W. (Eds.). Métodos alternativos de controle fitossanitário, Jaguariúna, CNPMA Embrapa, 2003. 279p.

BETTIOL, W. Seleção de microrganismos antagônicos a fitopatógenos. In: Bettiol, W. (Ed.) Controle Biológico de Doenças de Plantas. Jaguariúna, EMBRAPA, 388p, 1991.

CHEN, G.; DUNPHY, G.B.; WEBSTER, J.M. Antifungal activities of two Xenorhabdus species and Photorhabdus luminescens, bacteria associated with the nematodes Steinernema species and Heterorhabditis megidis. Biological Control, Amsterdam, 4: 157-162, 1994.

DEMANT, C. A. R. Mofo branco e seu manejo no oeste baiano. Boletim Passarela da Soja, 2. Luis Eduardo Magalhães, Fundação Bahia, 32p, 2010.

DI FIORE, S.; DEL GALLO, M. Endophytic bacteria: their possible role in the host plants. In: FENDRIK, I.; DEL

GALLO, M.; VANDERLEYDEN, J.; DE ZAMAROCZY, M. (Eds.) Azospirillum VI and related microrganisms. Berlin, Springer-Verlag, p.169-187, 1995.

EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária. Ocorrência de doenças causadas por Sclerotinia sclerotiorum em girassol e soja. Londrina, Ministério da Agricultura e Abastecimento, 2005. (Comunicado Técnico 76).

FERRARI, E.; VALIATI, S.; SHIOMI, H.F. Efeito de biofertilizantes no biocontrole de Penicillium sp. em laranja “pêra”. Scientific Electronic Archives, Sinop, 1: 1-5, 2012.

FORST, S.; DOWDS, B.; BOEMARE, N.; STACKEBRANDT, E. Xenorhabdus and Photorhabdus spp.: bugs that kill bugs. Annual Review of Microbiology, Palo Alto, 51: 47-72, 1997.

HIRASAWA, K.; UCHIMURA, K.; KASHIWA, M.; GRANT, W.; ITO, S.; KOBAYASHI, T.; HORIKOSHI, K. Salt-activated endoglucanase of a strain of alkaliphilic Bacillus agaradhaerens. Antonie van Leeuwenhoek, Amsterdam, 89 (2): 211-219, 2006.

HU, K.; WEBSTER, J.M. Antibiotic production in relation to bacterial growth and nematode development in Photorhabdus-Heterorhabditis infected Galleria mellonella larvae. FEMS Microbiology Letters, Amsterdam, 189: 219-223, 2000.

JACCOUD FILHO, D.S.; MANOSSO NETO, M.O.; VRISMAN, C.M.; HENNEBERG, L.; GRABICOSKI, E.M.G.; PIERRE, M.L.C.; BERGER NETO, A.; SARTORI, F.F.; DEMARCH, V.B.; ROCHA, C.H. Análise, Distribuição e Quantificação do “Mofo Branco” em Diferentes Regiões Produtoras do Estado do Paraná. Resumos... Reunião de Pesquisa de Soja da Região Central do Brasil, Brasília, Embrapa-Soja, 31, p.226-228, 2010.

KUPPER, K.C.; BETTIOL, W.; GÓES, A.; SOUZA, P.S.; BELLOTTE, J.A.M. Biofertilizer for control of Guignardia citricarpa, the causal agent of citrus black spot. Crop Protection, Guilford, 25: 569-573, 2006.

LI, D.; SUN, M.; HAN, Y.; TENG, W.; LI, W. Identification of QTL underlying soluble content in soybean content in soybean white mold (Sclerotinia sclerotiorum). Euphytica, Wageningen, 172: 49-57, 2010.

MANTOVANELLO, C.M.; MELO, I.S. Isolamento e seleção de rizobactérias promotoras de crescimento de plantas de tomate (Lycopersicon esculentum). Summa Phytopathologica, Botucatu, 20 (2): 123-126, 1994.

MARIANO, R.L.R. Métodos de seleção in vitro para o controle microbiológico de patógenos de plantas. In: LUZ, W.C. Revisão Anual de Patologia de Plantas, Passo Fundo, 1:369-409, 1993.

MARROCOS, S.T.P.; NOVO JUNIOR, J.; GRANGEIRO, L.C.; AMBRÓSIO, M.M.Q.; CUNHA, A.P.A. Composição química e microbiológica de biofertilizantes em diferentes tempos de decomposição. Revista Caatinga, Mossoró, 25 (4): 34-43, 2012.

MARTINEZ, C.; MICHAUD, M.; BELANGER, R.R.; TWEDDELL, R.J. Identification of soils suppressive against Helminthosporium solani, the causal agent of potato silver scurf. Soil Biology and Biochemistry, Elmsford, 34: 1861-1868, 2002.

MEDEIROS, E.J.; BULHÕES, C.C.; SANTOS, B.T.; SHIOMI, H.F. Ação antagônica de isolados bacterianos sobre Colletotrichum gloeosporioides em testes 'in vitro'. Tropical Plant Pathology, Brasília, 37 (Supl.), 2012a, 1 CD-ROM.

MEDEIROS, E.J.; BULHÕES, C.C.; SANTOS, B.T.; SHIOMI, H.F. Avaliação da ação antagônica de isolados bacterianos sobre Xanthomonas axonopodis pv. passiflorae 'in vitro'. Tropical Plant Pathology, Brasília, 37 (Supl.), 2012b, 1 CD-ROM.

MERHEJ, V.; ADÉKAMBI, T.; PAGNIER, I.; RAOULT, D.; DRANCOURT, M. Yersinia massiliensis sp. nov., isolated from fresh water. International Journal of Systematic and Evolutionary Microbiology, Reading, 58: 779-784, 2008.

OKA, N.; HARTEL, P.G.; FINLAY-MOORE, O.; GAGLIARDI, J.; ZUBERER, D.A.; FUHRMANN, J.J.; ANGLE, J.S.; SKIPPER, H.D. Misidentification of soil bacteria by fatty acid methyl ester (FAME) and BIOLOG analyses. Biology and Fertility of Soils, Berlin, 32: 256-258, 2000.

PERNDERGRASS, S.M.; JENSEN, P.A. Application of the gas chromatography-fatty acid methyl ester system for the identification of environmental and clinical isolates of the Family Micrococcaceae. Applied Occupational and Environmental Hygiene, Philadelphia, 12 (8): 543-546, 1997.

RYDER, M.H.; YAN, Z.; TERRACE, T.E.; ROVIRA, A.D.; TANG, W.H.; CORRELL, R.L. Use of strains of Bacillus isolated in China to suppress take-all and Rhizoctonia root rot, and promote seedling growth of glasshouse-grown wheat in Australian soils. Soil Biology and Biochemistry, Elmsford, 31: 19-29, 1999.

SAFDI, N.; CHÉRIF, M.; HAJLAOUI, M.R.; BOUDABBOUS, A. Biological control of the potato tubers dry rot caused by Fusarium roseum var. sambucinum under greenhouse, field and storage conditions using Bacillus spp. isolates. Journal of Phytopathology, Berlin, 150: 640–648, 2002.

SANTOS, A.C.V. Biofertilizante líquido, o defensivo da natureza. Niterói, Emater-Rio, 1992. (Agropecuária Fluminense, 8)

SHIOMI, H.F.; SILVA, H.A.S.; MELO, I.S.; NUNES, F.V.; BETTIOL, W. Bioprospecting endophytic bacteria for biological control of coffee leaf rust. Scientia Agricola, Piracicaba, 63 (1): 32-39, 2006.

SILVA, H.A.S.; ROMEIRO, R.S.; MACAGNAN, D.; HALFELD-VIEIRA, B.A.; PEREIRA, M.C.B.; MOUNTEER, A. Rhizobacterial induction of systemic resistance in tomato plants: non-specific protection and enzyme activities. Biological Control, Amsterdam, 29: 288-295, 2004.

SIMON, H.M.; SMITH, K.P.; DODSWORTH, J.A.; GUENTHNER, B.; HANDELSMAN, J.; GOODMAN, R.M. Influence of tomato genotype on growth of inoculated and indigenous bacteria in the spermosphere. Applied and Environmental Microbiology, Washington, 67: 514-520, 2001.

SRINIVASAN, M.; HOLL, F.B.; PETERSEN, D.J. Influence of indoleacetic-acid-producing Bacillus isolates on the nodulation of Phaseolus vulgaris by Rhizobium etli under gnobiotic conditions. Canadian Journal of Microbiology, Ottawa, 42 (10): 1006-1014, 1996.

SULAKVELIDZE, A.; KREGER, A.; JOSEPH, A.; ROBINS-BROWNE, R.M.; FASANO, A.; DE TOLA, L.; MORRIS JR, G. Production of enterotoxin by Yersinia bercovieri, a recently identified Yersinia enterocolitica-like species. Infection and Immunity, Washington, 62 (2): 968-971, 1999.

TRATCH, R.; BETTIOL, W. Efeito de biofertilizantes sobre o crescimento micelial e germinação de esporos de alguns fungos fitopatogênicos. Pesquisa Agropecuária Brasileira, Brasília, 32: 1131-1139, 1997.

VALIATI, S.; FERRARI, E.; SHIOMI, H.F. Efeito de isolados bacterianos no biocontrole “in vitro” de Aspergillus sp. Scientific Electronic Archives, Sinop, 1:11-15, 2012.

VALOIS, D.; FAYAD, K.; BARBASUBIYE, T.; GARON, M.; DÉRY, C.; BRZEZINSKI, R.; BEAULIEU, C. Glucanolytic actinomycetes antagonistic to Phytophthora fragariae var. rubi, the causal agent of raspberry root rot. Applied and Environmental Mycrobiology, Washington, 62: 1630-1635, 1996.

XIUJUN, Z.; SHIXIANG, W.; YI, Z.; QI, Z. Screening biocontrol agents for Pythium-induced disease by germinating carrot seeds in Petri dish – a rapid and effective method. In: WENHUA, T.; COOK, J.R.; ROVIRA, A. Advances in Biological Control of Plant Diseases. Beijing: China Agricultural University Press, p.140-144, 1996.


Apontamentos

  • Não há apontamentos.